Description
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with Avidin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody and Horseradish Peroxidase (HRP) -conjugated antibody preparation specific for HE4 and incubated. Then substrate solutions are added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of HE4 in the samples is then determined by comparing the O.D. of the samples to the standard curve